UJI AKTIVITAS ANTIBAKTERI FRAKSI N-HEKSANA DAN ETIL ASETAT DAUN JERUJU (Acanthus ilicifolius L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus

Azzahra, Fatimah (2025) UJI AKTIVITAS ANTIBAKTERI FRAKSI N-HEKSANA DAN ETIL ASETAT DAUN JERUJU (Acanthus ilicifolius L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus. D3-D4 thesis, Universitas Muhammadiyah Magelang.

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Abstract

ABSTRAK
Fatimah Azzahra, UJI AKTIVITAS ANTIBAKTERI FRAKSI ETIL ASETAT DAN HEKSANA DAUN JERUJU (ACANTHUS ILICIFOLIUS L) TERHADAP PERTUMBUHAN BAKTERI STAPHYLOCOCCUS AUREUS
Latar belakang: Meningkatnya resistensi antibiotik disebabkan oleh penggunaan antibiotik yang sering dan berkepanjangan sehingga penemuan agen antibakteri baru yang efektif sangat penting karena resistensi antibiotik semakin meningkat. Penelitian bahan alam sebagai sumber agen antimikroba potensial terus meningkat, terutama pada tanaman yang telah digunakan dalam pengobatan tradisional yaitu daun jeruju (Acanthus ilicifolius) sebagai alternatif antibakteri. Tujuan: Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri fraksi n-heksana dan etil asetat daun jeruju terhadap Staphylococcus aureus serta golongan senyawa dalam fraksi teraktif yang berperan paling signifikan dalam aktivitas tersebut. Metode: Ekstraksi daun jeruju dilakukan dengan teknik maserasi menggunakan pelarut etanol kemudian dilakukan fraksinasi cair-cair dengan menggunakan n-heksan dan etil asetat. Fraksi dilakukan proses KLT dan uji aktivitas antibakteri. Hasil: Fraksi n-heksana mengandung senyawa terpenoid, sedangkan fraksi etil asetat mengandung alkaloid, flavonoid, terpenoid, dan saponin. Fraksi etil asetat menunjukkan aktivitas antibakteri paling tinggi pada konsentrasi 7% dengan diameter zona hambat rata-rata 10,65 ± 0,65 mm, sebanding dengan kontrol positif (kloramfenikol) yaitu 11,44 ± 0,21 mm. Kesimpulan: Hasil penelitian menunjukkan bahwa fraksi etil asetat lebih efektif menghambat pertumbuhan Staphylococcus aureus dengan rata-rata zona hambat 10,65 ± 0,65, sebanding dengan kloramfenikol (11,44 ± 0,21 mm). Skrining fitokimia mengungkapkan bahwa fraksi ini mengandung flavonoid, alkaloid, saponin dan terpenoid yang berpotensi sebagai senyawa antibakteri.
Kata kunci: Daun jeruju (Acanthus ilicifolius), diameter zona hambat, antibakteri, Staphylococcus aureus
ABSTRACT
Fatimah Azzahra, ANTIBACTERIAL ACTIVITY TEST OF ETHYL ACETATE AND HEXANE FRACTIONS OF JERUJU LEAVES (ACANTHUS ILICIFOLIUS) ON THE GROWTH OF STAPHYLOCOCCUS AUREUS BACTERIA
Background: Increasing antibiotic resistance is caused by frequent and prolonged use of antibiotics, so the discovery of new effective antibacterial agents is very important because antibiotic resistance is increasing. Research on natural materials as a source of potential antimicrobial agents continues to increase, especially in plants that have been used in traditional medicine, namely jeruju leaves (Acanthus ilicifolius) as an alternative antibacterial. Objective: This study aims to determine the antibacterial activity of n-hexane and ethyl acetate fractions of jeruju leaves against Staphylococcus aureus and the group of compounds in the most active fraction that play the most significant role in this activity. Method: Jeruju leaves were extracted using maceration techniques using ethanol solvents, then liquid-liquid fractionation was carried out using n-hexane and ethyl acetate. The fractions were subjected to TLC and antibacterial activity tests. Results: The n-hexane fraction contains terpenoid compounds, while the ethyl acetate fraction contains alkaloids, flavonoids, terpenoids, and saponins. The ethyl acetate fraction showed the highest antibacterial activity at a concentration of 7% with an average inhibition zone diameter of 10.65 ± 0.65 mm, comparable to the positive control (chloramphenicol) which was 11.44 ± 0.21 mm. Conclusion: The results showed that the ethyl acetate fraction was more effective in inhibiting the growth of Staphylococcus aureus with an average inhibition zone of 10.65 ± 0.65, comparable to chloramphenicol (11.44 ± 0.21 mm). Phytochemical screening revealed that this fraction contains flavonoids, alkaloids, saponins and terpenoids which have the potential as antibacterial compounds.
Keywords: Jeruju leaves (Acanthus ilicifolius), phytochemical screening, inhibition zone diameter, antibacterial, Staphylococcus aureus

Item Type:	Karya Ilmiah (D3-D4)
Pembimbing:	apt. Puspita Septie Dianita, S.Farm,. M.PH dan apt. Alfian Syarifuddin, M. Farm
Uncontrolled Keywords:	pemutusan hubungan kerja, tata cara, akibat hukum
Subjects:	R Medicine > R Medicine (General) R Medicine > RS Pharmacy and materia medica
Depositing User:	FIK 22.0602.0005
Date Deposited:	22 Sep 2025 02:00
Last Modified:	22 Sep 2025 02:01
URI:	https://repositori.unimma.ac.id/id/eprint/5847

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